thruplex fragmented dna prep kit thruplex-fd  (Rubicon Genomics)

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Two DNA samples (Test DNA 1 and Test DNA 2) were subjected to four exome sequencing (ES) protocols performed in parallel: control (Standard ES) and three modified (REPLI-g ES, GenomePlex ES and ThruPLEX-FD ES). Common steps performed in parallel for several protocols are shown by text boxes spanning the corresponding number of protocol columns.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques: Sequencing, Control, Modification

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Alignment statistics.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques:

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Coverage statistics for the target region.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques:

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Pearson correlation coefficient with coverage profile of Standard ES strategy and average deviation from Standard ES coverage profile.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques:

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Only variation with minimum depth of coverage of 20x and minimum quality of 13 were taken into account in all four strategies. The names of the samples are abbreviated: Standard ES = St; ThruPLEX-FD ES = Tp; REPLI-g ES = Rg; GenomePlex ES = Gp. The lower left tile presents the overall statistics, where “Total” indicates the number of all unique SNVs found in the region of interest, i.e. the union of SNV sets found by each strategy.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques:

Journal: PLoS ONE

Article Title: Exome Sequencing from Nanogram Amounts of Starting DNA: Comparing Three Approaches

doi: 10.1371/journal.pone.0101154

Figure Lengend Snippet: Comparison of SNVs found in regions with coverage > = 20 in both Standard ES and one of three tested strategies.

Article Snippet: One library was prepared starting from 10 ng of sheared original DNA using ThruPLEX Fragmented DNA Prep kit (ThruPLEX-FD) from Rubicon Genomics (Cat. No. R40012).

Techniques: Comparison